Sull’ultimo numero della rivista Cytokine, Ferreira et al. hanno pubblicato una serie di esperimenti volti a verificare l’effetto di Phosphorus in diluizione ultramolecolare,

in seguito all’infezione, provocata in laboratorio sul ratto, del Trypanosoma cruzi, portatore della malattia di Chagas. La malattia di Chagas è molto diffusa nell’America centrale e del sud, e si stima che uccida 12.500 persone all’anno.  I sintomi cambiano nel corso dell'infezione. Nella fase iniziale, generalmente essi non sono presenti o si rivelano lievi, come febbre, ingrossamento dei linfonodi, mal di testa e gonfiore locale sul sito di puntura. Dopo 8-12 settimane, gli individui entrano nella fase cronica della malattia e nel 60%-70% non si presentano altri sintomi. Nel restante 30%-40% si sviluppano ulteriori sintomi in un periodo tra i 10 e i 30 anni dopo l'infezione iniziale, compreso l'allargamento dei ventricoli del cuore nel 20%-30% dei casi, portando ad insufficienza cardiaca. Nel 10% dei pazienti si può osservare anche un allargamento dell'esofago o del colon[1]. Non esiste una cura effettiva e neppure un vaccino. Gli scienziati hanno infettato un gruppo di ratti con il tripanosoma e successivamente ha fornito Phosphorus 13CH. In seguito ad analisi del sangue e monitoraggio del tessuto cardiaco, gli scienziati hanno verificato che i topi che avevano ricevuto il Phosphorus mostravano meno foci nel tessuto cardiaco ed un aumento dei livelli di interferone-g e TNF-a. L’insieme di questi importanti risultati orienta senz’altro la ricerca sull’utilizzo primario della Medicina Omeopatica in questa feroce e subdola malattia tropicale.

 

[1] Wikipedia

 

Cytokine. 2017 Jul 27. pii: S1043-4666(17)30217-X. doi: 10.1016/j.cyto.2017.07.017.

Phosphorus protects cardiac tissue by modifying the immune response in rats infected by Trypanosoma cruzi.

Ferreira ÉCCiupa LPortocarrero ARBrustolin CFMassini PFAleixo DLde Araújo SM.

 

Abstract

AIM:

This study evaluates and correlates the number of myocarditis focuses and production of cytokines in Rattus norvegicus (Wistar lineage), experimentally infected with T. Cruzi and treated with Phosphorus.

METHODS:

In two blind, controlled and randomized trials, 53 45-day-old, male animals were allocated into groups Control (n=24): Control group infected and treated with 7% hydroalcoholic solution, the preparation vehicle of the test medication; and Phosphorus (n=24 on days 0, 5, 10 and 24 after infection): group infected and treated with Phosphorus 13cH, diluted 10-26 and dynamized (test medication). The animals were inoculated intraperitoneally with 5×106 blood trypomastigotes of T. cruzi-Y strain. The medication was administered overnight (16 consecutive hours), diluted in water (1mL/100mL) in amber water bottles. The animals were treated 2days before and 2, 4, and 6days after infection. Enumeration of inflammatory foci in cardiac tissue (Hematoxylin-Eosin) and dosage of cytokines TNF-α and IFN-γ in the serum were performed on days 0, 5, 10 and 24 after infection, using three animals/group. Mann-Whitney, Friedman ANOVA, Spearman correlation (p<0.05), and Statistica Single User Software version 13.2 were used for data analysis.

RESULTS:

The animals treated with Phosphorus 13cH had high concentration of INF-ɣ on the 5th day of infection with significant decrease on the 10th and 24th days (p<0.05), and high concentration of TNF-α on the 5th and 10th days of infection with decrease on the 24th day (p<0.05). The treatment with Phosphorus caused a significant increase of INF-ɣ and TNF-α on the 5th day of infection compared with the Control (p<0.05), with reestablishment on the 24th day, as well as in the Control group. The group treated with Phosphorus had 52.5% less number of myocarditis focuses in heart than Control group (p<0.05) on the 10th day of infection. The significant increase in cytokines on the5th day of infection in the Phosphorus group is related to a significant decrease in the number of inflammatory foci in cardiac tissue on the 10th day of infection in this group.

DISCUSSION AND CONCLUSION:

Treatment with Phosphorus 13cH promotes beneficial effects in T. cruzi infection in Wistar rats by modulating the secretion of IFN-γ and TNF-α with decreased inflammation in cardiac tissue. These results reinforce the importance of considering the use of homeopathy for establishing new therapeutic approaches in the management of patients with Chagas disease.